BET Water is used to fulfill a variety of endotoxin detection assays.
Ø In gel clot method BET assay to reconstitute both CSE and LAL Reagent;
Ø In quantitative BET assay to reconstitute both CSE and LAL Reagent for the creation of standard curves;
Ø Making of product dilutions;
Ø BET water provides reliable and reproducible data when paired with endotoxin-specific LAL reagents.
BETW10: 10ml vial, endotoxin-free standard<0.001EU/ml.
BET Water is used to fulfill a variety of endotoxin detection assays.
Ø In gel clot method BET assay to reconstitute both CSE and LAL Reagent;
Ø In quantitative BET assay to reconstitute both CSE and LAL Reagent for the creation of standard curves;
Ø Making of product dilutions;
Ø BET water provides reliable and reproducible data when paired with endotoxin-specific LAL reagents.
BETW10: 10ml vial, endotoxin-free standard<0.001EU/ml.
LAL Reagent Water ( Water for Bacterial Endotoxins Test )
1. Product Information
LAL Reagent Water (Water for Bacterial Endotoxins Test or BET Water or Water for BET ) is specially processed super-purified endotoxin free water is used for Endotoxin test. Its endotoxin concentration is less than 0.005 EU/ml. Various packages, such as 2ml, 10ml, 50ml, 100ml and 500ml per unit, are provided for users’ convenience. LAL Reagent Water (Water for BET) could be used to dilute the assay sample, construct the stand curve, or reconstitute the Lyophilized Amebocyte Lysate reagents.
2. Product Parameter
Endotoxin level: ≤0.005 EU/ml
Water for Bacterial Endotoxins Test is specially processed water is used for endotoxin detection. Its endotoxin concentration is less than 0.005EU/ml. We also provide water for BET with endotoxin level less than 0.001EU/ml for the assay sensitivity 0.001 to 5EU/ml kinetic chromogenic assay.
3. Product Features and Application
Endotoxin Free Water (Water for BET, LAL Reagent water, Endotoxin free water or BET water) is specially processed water intended for reconstitution of Lyophilized Amebocyte Lysate and Control Standard Endotoxin (CSE), and to dilute samples and control standards in the endotoxin assay operation.
For reconstitution of Lyophilized Amebocyte Lysate or Amebocyte Lysate, dilution of the test samples and Control Standard Endotoxin, preparation endotoxin free buffers, and construction of the standard curve. 500ml BET water main apply in extract endotoxins from medical devices.
Catalog No. | Volume (ml/vial) | Package |
TRW02 | 2ml in Ampoule | In Ampoule, 10 Ampoules/Pack |
TRW05 | 5ml in Ampoule | In Ampoule, 10 Ampoules/Pack |
TRW10 | 10ml in Ampoule | In Ampoule, 10 Ampoules/Pack |
TRW50 | 50ml in Glass Vial | In Glass Vial, 10 Vials/Pack |
TRW100 | 100ml in Glass Vial | In Glass Vial, 10 Vials/Pack |
TRW500 | 500ml in Plastic Bottle | 1 Bottle |
The sensitivity of Lyophilized Amebocyte Lysate and potency of Control Standard Endotoxin are assayed against USP Reference Standard Endotoxin.
The Lyophilized Amebocyte Lysate reagent kits come with product instruction, Certificate of Analysis.
Purified water with sterilization and depyrogenation treatment.
These key points highlighting the importance of LAL reagent water in the different steps of endotoxin testing:
Reconstitution of LAL Reagent and CSE :
The Control Standard Endotoxin (CSE) and Lyophilized Amebocyte Lysate (LAL) reagents used in the test are often lyophilized or freeze-dried to ensure stability. Reconstituting the two item with BET water (LAL reagent water) is a critical step to activate the LAL and prepare the standard solutions needed for the LAL test assay.
Creation of Standard Curves in Quantitative Methodological Principle:
Standard curves are essential for quantifying the amount of endotoxin present in a sample. BET water (LAL reagent water) is used to create dilutions of the CSE, which are then used to generate a standard curve. The accuracy of the standard curve is crucial for determining the endotoxin concentration in test samples.
Product Dilutions:
When testing samples for endotoxin content, it's often necessary to dilute the samples to ensure that the endotoxin concentration falls within the range of the standard curve. BET water (LAL reagent water) is used in the dilution process to maintain the accuracy of the test results.
Control Samples:
In addition to the standards and samples, control samples are often included in the testing process to validate the performance of the LAL reagents and the test assay system. BET water (LAL reagent water) is used to prepare control samples, ensuring that any detected endotoxin is attributed to the sample being tested and not the water used.
Reliability and Reproducibility:
Using consistent and high-quality BET water (LAL reagent water) contributes to the reliability and reproducibility of the endotoxin test results. Variability in the water quality could introduce uncertainties and affect the accuracy of the assay.
BET water (LAL reagent water) is a critical component in the Bacterial Endotoxin Test, and its quality directly impacts the accuracy and reliability of the endotoxin testing process. It ensures proper reconstitution of reagents, accurate standard curves, reliable product dilutions, and overall consistency in the test results. Choosing a water source specifically designed and tested for endotoxin-free conditions is essential for achieving valid and meaningful endotoxin test outcomes.
Jeffrey - (0086) 181 0176 4785 Wechat/Whatsapp: (0086) 181 0176 4785 Email: sales@biocelgene.tech
Grace - (0086) 189 6254 1125 Wechat/Whatsapp: (0086) 189 6254 1125 Email: grace@biocelgene.tech